Glycolysis and Anaerobic Respiration

Glycolysis (it literally means glucose degradation) is a metabolic pathway which changes glucose C6H12O6 into pyruvate CH3COCOO + H. The free energy which is generated in this process is utilized to make high energy compounds namely ATP (adenosine triphophate) and NADH (reduced nicotinamide adenine dinucleotide).
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Equation of anaerobic respiration

Respiration is a process which happens inside the cells in which carbohydrates, especially glucose, is broken down for the energy to be released which can be used by the cells. This energy generated can be used for a lot of different processes but in all of those processes energy is transferred. The processes involved range from muscle contraction to the production of protein for the new cells. This respiration is an important feature of life. This is carried out by all the living cells. There are two varieties or types of respiration in organisms-aerobic and anaerobic. The aerobic respiration is a complicated procedure involving chemical reactions in which oxygen is used to transform glucose into carbon dioxide and H2O. This process generates energy in the form of energy carrying molecules called as ATP. At other times the respiration takes place without oxygen, this is termed as anaerobic respiration. In the case of anaerobic respiration, glucose is broken and the products generated from this are energy and either lactic acid or ethanol (alcohol) and CO2. This process is termed as fermentation.
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Anaerobic Respiration: Fermentation

Anaerobic respiration is a type of respiration in which foodstuffs (normally carbohydrates) are partly oxidized with the chemical energy released, and in the process there is no involvement of atmospheric oxygen. As the substrate is never totally oxidized the energy generated of this type of respiration is lesser than that generated during aerobic respiration. This occurs in some yeasts and bacteria and in the muscle tissue when oxygen is not present. This is also called as oxygen debt. The obligate anaerobes are the organisms which cannot use the atmospheric oxygen for respiration. Facilitative anaerobes are mostly aerobic but can respire anaerobically in the period of oxygen shortage. Alcoholic fermentation is a type of anaerobic respiration in which one of the products of the reaction is ethanol.
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Anaerobic Respiration in Humans

An anaerobic organism also called as anaerobe is an organism which does not require oxygen for its growth and may even lose its life in the presence of oxygen. There are three types of anaerobe: obligate anaerobe, which do not use oxygen for growth and even get harmed by it; aero tolerant organisms which cannot use oxygen for their growth but are able to sustain in its presence; and facultative anaerobes which can grow without the presence of oxygen and also can utilize its presence.
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Anaerobic Respiration in Yeast

In biology the term anaerobic respiration means a way for a life form to generate usable energy without involvement from oxygen. So in brief it is respiration without oxygen. Respiration is a redox reaction which processes energy in a form which is usable by a life form, mainly a process of generating ATP the universal energy currency of life. Anaerobic respiration however should not be confused with fermentation like say ethanol fermentation. The commercial applications of anaerobic respiration include anaerobic digestion and mechanical biological treatment. These applications are used for waste water treatment.
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Anaerobic Digestion and Its Uses

Anaerobic digestion means the breaking down of the organic wastes and other materials through natural means. This breaking down occurs due to anaerobic respiration and results in the production of methane, fertilizers and carbon dioxide gas. The breaking of waste materials may either happen through natural means, or it can be done manually in an anaerobic digester specially built for this purpose.

A digester where anaerobic digestion will take place is usually a sealed vessel or vessels where the bacteria can act without the need of oxygen. The organic waste materials should be mixed fully and kept warm, generally equivalent to the blood temperature of the human body. The process of digestion starts with anaerobic respiration and bacterial hydrolysis of the organic waste materials that starts to break these into organic polymers and carbohydrates, which are insoluble. The other bacteria that is produced, takes over and converts the sugar and amino acids into carbon dioxide, ammonia, organic acids and hydrogen. The Acetogenic bacteria in the materials then take over and convert the organic acids into acetic acid.

During the process of digestion through anaerobic respiration, biogas, which is actually the name given to a mixture of gasses that gets formed during anaerobic digestion of the wastes, is produced. This biogas is composed of 70 percent methane and 30 percent of carbon dioxide. The biogas that is obtained can be used in fixed engines to produce electricity. However, this gas is not suitable to be used as a fuel for vehicles.

Anaerobic digestion is useful for producing renewable energy, because during the process of digestion that takes place through anaerobic respiration, the gas (biogas) that is produced is very rich in methane and carbon dioxide and is highly suitable for producing alternative sources of energy and can effectively reduce our dependence on fossil fuels. The digestate, which is produced, is very rich in nutrients and can also be used in the place of chemical fertilizers.

Another byproduct is Biomethane, also known as the Renewable Natural Gas. Removing carbon dioxide as well as other gases produces this gas. This process is known as the upgrading of gas. The Biomethane Gas obtained is similar to the natural gas. The only difference is that Biomethane Gas can be obtained very quickly, whereas, natural gas is produced through natural means after millions of years.

Biomethane Gas can be used in place of natural gas. It can be easily used for heating, cooing and can also be used as a source for the production of a variety of chemicals, hydrogen or fertilizers. This gas is used as a fuel for vehicles.

Anaerobic digesters can also be used effectively for waste management, as the anaerobic respiration that is induced during the digestion process also reduces the emission of harmful gases into our atmosphere. This type of digestion is specially suited to organic material wastes that are wet and are found in the sewage systems.

Most of the organic waste materials can be easily processed with anaerobic digestion. These materials include waste materials such as waste paper, leftover food, animal waste, sewage, grass clippings and the host of other materials that can be digested through anaerobic respiration.

What are the Differences Between Anaerobic Respiration and Anaerobic Fermentation?

If we compare both anaerobic respiration and anaerobic fermentation, we can say that both of them are completely separate from each other as both follow different pathways. In anaerobic respiration, the process involves the transfer of electrons through a system of chain system in the membrane of the cell.

In this way anaerobic respiration is quite similar to aerobic respiration. In this type of respiration, the process goes through glycolysis, oxidation of the pyruvate, Kerb cycle and then ultimately the transfers the chain of electrons, just like the aerobic respiration. The only difference between these two types of respiration is that anaerobic respiration do not need oxygen (nitrite, nitrate etc) while in aerobic respiration, oxygen is absolutely necessary.

Fermentation takes place when a co-enzyme, NADH reduces the pyruvate to form the organic compound. It is the process of getting energy by the oxidation of some compounds like carbohydrates, and by using an endogenous electron-acceptor that is usually an organic compound. The common products of fermentation are lactic acid, ethanol and hydrogen etc. But respiration is the process where electrons are given to an exogenous electron-acceptor, such as oxygen, through the electron transport chain. Another difference between fermentation and respiration is that it is not necessary for fermentation to occur in environments suitable for anaerobic respiration. A good example of this is yeast, which ferments even if oxygen is present, or if sugar is present. Thus, we can easily say that fermentation can occur when the electrons that are present in the coenzymes (NADH derived after glycolosis) are turned back partly to pyruvate. It should also be noted that the electrons are donated to things that have come from the pyruvate cells.

Fermentation is processed through the following path: Glycolysis – then donating the electron back to the pyruvate or product of pyruvate (which is electron or accepter coming from the internal source). However, anaerobic respiration proceeds through the following path: Glycolysis – oxidation of the pyruvate – Kerb cycle – transfers chain of the electron, which has the electron acceptor at the terminal end (without the requirement of oxygen).

Fermentation also occurs in some muscle cells, which are also called twitch muscles, because these muscles cannot store or use much oxygen in comparison to the other muscles. When we run the oxygen, supply of these muscles gets short as a result of which the twitch muscles starts using the fermentation of lactic acid. Through this process, the muscles can go on functioning as ATP is produced by the Glycolysis.

There is yet another difference between anaerobic respiration and anaerobic fermentation is the electron acceptor, which is also known as the final electron acceptor. For fermentation, pyruvate is the final electron acceptor. This can be seen in yeast fermentation, which gives alcohol as the final acceptor and does not break further for releasing energy. However, the main purpose of anaerobic respiration is to produce ATP (Adenosine Triphosphate), which is used by a cell for energy purposes.

Anaerobic Respiration

Respiration occurs in two ways, aerobic respiration that uses oxygen to function and anaerobic respiration which functions without oxygen. Anaerobic respiration start by breaking down the molecules of glucose and produces pyruvic acid. This acid then ferments and produces ATP, which is used by the cells for producing energy. One good thing is that human beings are able to perform both types of respiration.

There are some plants and animals, which can use anaerobic respiration also, but only for a short period of time. This is possible especially during running or sprinting when the muscles use anaerobic respiration. Whenever we perform intense physical exercises, our muscles use anaerobic respiration and produces lactic acid. The production of lactic acid and its buildup is the main reason why our muscles become weak and pain after exertion.

The anaerobic respiration is the oldest method of cellular respiration. Many single celled primitive organisms, which inhabit in places and environments lacking oxygen, such as the muddy bottom of a river, use this form of respiration for living.

This type of respiration primarily works by fermentation, which is also known as glycolysis. In this process, one glucose molecule is divided into two pyruvic acid molecules and acquires two molecules of ATP.  Then these ATP’s are used for splitting a molecule of glucose into two chains, each consisting of three numbers of a carbon atom. Both chains have one-phosphate groups at their end. Continuing the process, a different phosphate group gets itself attached to each of the three carbon chains. As a result, both of the phosphate groups on every chain are divided equally amongst the two molecules, which are known as ADP (Adenosine Diphosphate) and then turn into ATP.

During anaerobic respiration carbohydrates are partly oxidized and chemical energy is released without the requirement of oxygen. Some yeasts and bacteria and some muscle tissue also uses anaerobic respiration. Fermentation of the alcohol, which produces ethanol, is a perfect example of anaerobic respiration. This is the basis of the production of alcohol. The main purpose of anaerobic respiration is to produce ATP (Adenosine Triphosphate), which a cell uses for energy purposes.

Though this process of respiration is less efficient in producing energy, because it produces only two ATP molecules in comparison to 38 molecules produced during aerobic respiration. Aerobic respiration is in reality a faster process. This process is also used in making of breads, where the anaerobic respiration of the yeasts helps the bread to rise.

The anaerobic respiration conducted by the cells give rise to lactic acid, a chemical that actually helps in burning our muscles, if we do physical labor for a short time. Through this process of respiration, certain microorganisms throw out ethyl alcohol and carbon dioxide, which are actually waste products.

This form of respiration is very primitive and had started from the time or period when oxygen was missing in our atmosphere. Many living organisms have successfully adapted to anaerobic form of respiration to survive especially in environments and habitats that are not suitable for life due to insufficient oxygen or in the places that are totally lacking in it.

Simple Definition of Aerobic and Anaerobic Respirations

All living creatures, be it man or the smallest bacteria have one function in common, which is known as respiration. During respiration, two important functions are performed in living things. In the first, electrons that were generated during the catabolism are disposed off and in the second, ATP (also known as adenosine tri-phosphate) is produced. Respiration is conducted from the cell membranes through the prokaryotes, which is found inside the mitochondria.

There are two types of respiration (i) aerobic respiration and (ii) anaerobic respiration. While aerobic respiration is a process that requires oxygen, but in anaerobic respiration, oxygen is not required. Therefore aerobic and anaerobic respiration differs in terms of the amount of energy that is produced.

In aerobic respiration, oxygen is always required. During the process of aerobic respiration, the molecules of food are broken down to obtain energy. Oxygen is present at the end point of the electron acceptor. The molecules of fuel that are generally used by the cells of the body during the process of the respiration are composed of glucose, fatty acids and amino acids. In other words, when there is respiration through the aerobic process, Glucose together with Oxygen produces Energy, Carbon Dioxide and Water.

A lot of energy is produced during aerobic respiration, which may be as high as 38 ATP molecules for every glucose molecule. This means that during aerobic respiration only one molecule of glucose will produce about thirty-eight energy units.

In plain language, anaerobic means where there is no air and thus anaerobic respiration is a term used for the respiration that occurs without the use of oxygen. In this process, the molecules carry oxidation, when oxygen is absent. This results in the production if energy or ATP. This type of respiration is also equivalent to fermentation when energy production path (Glycolytic pathway) is functioning in one cell. There are two processes of this respiration (i) alcoholic fermentation, where the Glucose gets broken down and produces Energy (ATP), Ethanol and Carbon Dioxide as well as (ii) Lactate fermentation where Glucose breaks itself into Energy and Lactic Acid.

Anaerobic respiration produces less energy when compared with the process of aerobic respiration. This can be best illustrated with the fact that during the anaerobic respiration only two molecules of ATP are yielded for one glucose molecule

Anaerobic respiration produces less energy in comparison to aerobic respiration. When the anaerobic respiration (alcoholic fermentation) as at one above functions, only two molecules of ATP are given out for one glucose molecule, while for lactate fermentation as at two above, 2 ATP molecules are given out for each glucose molecule used during the process. Thus, during anaerobic respiration, only one molecule of glucose is broken down to obtain only 2 ATP molecule.

Generally anaerobic respiration is always used by the primitive living organisms, which live in places where oxygen is missing, like muddy bottom of a river. In such places, the organism survives without depending to a large extent on oxygen. These habitats are known as anoxic.